Formalin-fixed paraffin-embedded tissue represents a valuable sample source for molecular cancer research. These samples provide a contextual snapshot of the tissue at a specific time point and stage of disease but are highly challenging to analyze, especially using next generation sequencing.
DNA derived from FFPE is oftentimes highly fragmented, cross-linked with protein and has a high proportion of single-stranded DNA. making it challenging for adaptor ligation and amplification, steps that are critical for successful preparation of sequencing libraries.
The Agilent NGS FFPE QC kit is a qPCR-based assay that enables functional DNA quality assessment of input DNA prior to preparation of next generation sequencing libraries.
This kit enables assessment of the integrity of DNA as well as accurate quantitation of amplifiable template going into library preparation. Furthermore, optimizations to the SureSelectXT workflows based on the sample quality score are enabled.